Abstract Background Eighty-three strains of Leuconostoc mesenteroides were isolated from Algerian raw camel milk.Based on morphological, biochemical, and physiological characters tests, strains were identified as Ln.mesenteroides subsp.mesenteroides.Seven strains had a remarkable antagonistic and probiotic characterization.
The present study aims at identifying these strains by means of 16 s Luminous smoothing Oil rRNA gene sequencing and Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), extending phenotypic and genotypic studies done previously.Results The phyloproteomic dendrograms of the studied strains based on MALDI-TOF MS provided the same identification with more intraspecific information from the 16S rRNA gene sequencing based on phylogenetic analysis.The latter were in agreement with the previous biochemical/physiological identification, the seven isolated strains were Ln.mesenteroides subsp.mesenteroides.
Conclusions Remarkably, MALDI-TOF MS BROWS SERUM fingerprinting was found to be effective enough as 16S rRNA gene sequencing identification, allowing faster and more reliable analysis than biochemical/physiological methods.